Reduction of bioreactorĬontamination by microorganisms is a key factor for The relatively high membrane permeability enables the useīioreactor Contamination. The virus barrierįilter can be autoclaved. Insulin and transferrin show high passage through the filter.

High densities, maintenance of cell viability, and productivity in expressing protein The filtered media are indistinguishable from unfiltered media in growth of cells to Robust removal occurs primarily by sizeĮxclusion as demonstrated over a wide range of feedstocks and operating conditions. LRV, the mycoplasma Acholeplasma laidlawii by g8.8 LRV, and the bacteriaīrevundimonas diminuta by g9.2 LRV. Of mice by >3 log reduction value (LRV), the 28 nm bacteriophage ΦX174 by >4.5 This filter removes the 20 nm minute virus Viruses from serum-free cell culture media. A novel virus barrier filter has been developed for removing The bioreactor through virus inactivation or removal steps, which act as viral barriersĪround the bioreactor. The highĮxpense of such a contamination can be reduced by passing all gases and fluids feeding Pharmaceutical products can become contaminated with adventitious viruses. Genentech, Inc., 1 DNA Way, South San Francisco, California 94080, and Millipore Corporation,īioprocess Division, 80 Ashby Rd., Bedford, Massachusetts 01730Ĭommercial bioreactors employing mammalian cell cultures to express biological or

Joann Vennari,† Kimberly Turco,‡ Robert Piper,‡,§ Robert Kiss,† and Herbert Lutz*,‡ Shengjiang Liu,† Mark Carroll,‡ Raquel Iverson,† Christine Valera,† Development and Qualification of a Novel Virus Removal Filter for

Adware removal tsa safe